Sub Nanogram Detection. High Signal to Noise Ratio. 3 Hour Protocol. Reversible. Multiple Imaging Options
AdvanStain Scarlet is based on epicocconone, a small, naturally occurring compound that only fluoresces when reversibly bound to protein via basic amino acids. The unbound dye does not fluoresce, resulting in very low backgrounds and no speckling. AdvanStain Scarlet is sensitive, allowing visualization of less than 1 ng of protein per band or spot. AdvanStain Scarlet also provides a large linear dynamic range for more accurate protein quantitation and reliable comparisons of protein amount between samples.
The AdvanStain Scarlet protocol can be completed in 3 hours, and stained gels or blots can be imaged under multiple imaging conditions including excitation with UV (302, 365 nm), violet (405 nm) blue (488 nm), and green (543, 532 nm) wavelengths, using CCD or laser-based imaging systems. AdvanStain Scarlet is fully compatible with downstream applications including mass spectrometry.
- Sensitive – detect less than a nanogram of protein per spot
- Convenient – simple 3-hour protocol
- Flexible – stain gels or membranes
- Reversible – destain for downstream Western blotting or mass spectrometry
- No speckling – clean background for better data
- Safe – biodegradable and contains no heavy metals for increased safety and simple disposal
- Compatible – image with any laser or CCD imaging system. Compatible excitation wavelenghts include green (543, 532 nm), blue (488 nm), violet (405 nm) and UV (302, 365 nm). Maximum emission wavelenght is 610 nm