• Lost sample to Gb
  • Lost extractions to bioinformatics
  • Lost amplicons to whole genomes

Long non-coding (Lnc) RNAs refer to RNAs of which length is greater than 200 nt and not involved in protein coding. LncRNAs have been characterized to be associated with important cellular processes such as X-chromosome inactivation, imprinting, maintainance of pluripotency, lineage commitment and apoptosis in mammalian system.

Project Workflow

lncRNA WorkflowFunctions of LncRNAs

lncRNA Function

(Wenqian H., et al., EMBO Reports. 2012)

Classification of LncRNAs

lncRNA Classification

(Cheng W., et al., Cancer Letters. 2013)

Sequencing Strategy

  • 200 bp insertion strand-specific cDNA library
  • HiSeq 2500 Platform V4 reagent, Paired-end 125 bp

Sample Requirements

  • Total RNA amount: ≥6 μg
  • RNA concentration: ≥100 ng/μL
  • RIN value ≥6.5 for plants and fungi; RIN value≥7.0 for animals
  • Purity: OD260/280 = 1.8-2.2, OD260/230 ≥ 2.0 without degradation and DNA contamination

Turnaround Time

  • Within 60 days from sample verification

Recommended Data Amount

  • 10 Gb clean data for general depth
  • 20 Gb clean data for high depth

Analysis Pipeline

lncRNA Analysis