RiboCop rRNA Depletion Kit (Human/Mouse/Rat)
After extraction total RNA comprises large amounts of ribosomal RNA (rRNA) which frequently is of little interest. Lexogen's RiboCop rRNA Depletion Kit enables removal of rRNA from total RNA and is suited for Next Generation Sequencing as well as other demanding RNA analysis applications.
Efficient Elimination of rRNA
RiboCop allows efficient removal of cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse and rat samples. Thus, sequencing depth is preserved for relevant RNA species.
The RiboCop protocol takes 2 hours to complete. No enzymatic reactions or mechanical shearing steps are involved, leaving full-length transcripts intact for downstream processing.
Low Input Amount
RiboCop was characterized down to input levels of 10 ng total RNA.
Magnetic beads are used for depletion and purification steps, rendering the protocol completely automation friendly.
RiboCop is offered in different kit sizes as a stand-alone kit (Cat. No. 037.24, 037.96) as well as in combination with SENSE Total RNA-Seq Libray Prep Kit (Cat. No. 042.08, 042.24, 042.96).